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Western Blot, Positive WB detected in: NIH/3T3 whole cell lysate, All lanes: RBMS1 antibody at 3.6ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 45, 5 kDa, Observed band size: 45 kDa
Immunofluorescence staining of HepG2 cells with CAC15116 at 1:120, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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