Western Blot, Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, All lanes: RAD51 antibody at 2.7ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 37, 27, 32 kDa, Observed band size: 37 kDa
Immunohistochemistry of paraffin-embedded human liver cancer using CAC14547 at dilution of 1:100
Immunofluorescent analysis of Hela cells using CAC14547 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating RAD51 in K562 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC14547 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC14547 (4ug) + K562 whole cell lysate (500ug), Lane 3: K562 whole cell lysate (20ug)