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Western Blot; Positive WB detected in: HepG2 whole cell lysate, A2780 whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate; All lanes: PRSS8 antibody at 2µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 37, 31 kDa; Observed band size: 70 kDa
Immunofluorescence staining of MCF-7 cells with CAC11252 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Safety Data Sheet