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Western Blot; Detected samples: Hela whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 10mM sodium propionate for 4h; All lanes: HIST1H4A antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa
Chromatin Immunoprecipitation Hela (4*106, treated with 10mM sodium propionate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CAC11483) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.

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