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Immunofluorescence staining of HepG2 cells with CAC08755 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; ,Positive WB detected in: HepG2 whole cell lysate; ,All lanes: Prf1 antibody at 4µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 63 kDa; ,Observed band size: 63 kDa

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