Western Blot, Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, All lanes: PPOX antibody at 2.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 51 kDa, Observed band size: 51 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CAC13879 at dilution of 1:100
Immunofluorescent analysis of PC-3 cells using CAC13879 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating PPOX in HepG2 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13879 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13879 (8ug) + HepG2 whole cell lysate (500ug), Lane 3: HepG2 whole cell lysate (10ug)