Western Blot, Positive WB detected in: Hela whole cell lysate, Rat kidney tissue, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue, All lanes: PPA2 antibody at 3.1ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 38, 40, 35, 19, 26 kDa, Observed band size: 38 kDa
IHC image of CAC15445 diluted at 1:200 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.