Poldip3 Polyclonal Antibody, Cat#CAC11219
Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate; All lanes: POLDIP3 antibody at 3.2µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 47, 43 kDa; Observed band size: 43 kDa;
Immunohistochemistry of paraffin-embedded human testis tissue using CAC11219 at dilution of 1:100
Immunofluorescence staining of Hela cells with CAC11219 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating POLDIP3 in Jurkat whole cell lysate; , Lane 1: Rabbit control IgG instead in Jurkat whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11219 (8µg) + Jurkat whole cell lysate (500µg); , Lane 3: Jurkat whole cell lysate (10µg);
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Poldip3 Polyclonal Antibody, Cat#CAC11219 Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate; All lanes: POLDIP3 antibody at 3.2µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 47, 43 kDa; Observed band size: 43 kDa; Immunohistochemistry of paraffin-embedded human testis tissue using CAC11219 at dilution of 1:100 Immunofluorescence staining of Hela cells with CAC11219 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating POLDIP3 in Jurkat whole cell lysate; , Lane 1: Rabbit control IgG instead in Jurkat whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11219 (8µg) + Jurkat whole cell lysate (500µg); , Lane 3: Jurkat whole cell lysate (10µg);

Poldip3 Polyclonal Antibody

BiomatikSKU: CAC11219-100ug
Size: 100ug
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Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate; All lanes: POLDIP3 antibody at 3.2µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 47, 43 kDa; Observed band size: 43 kDa;
Immunohistochemistry of paraffin-embedded human testis tissue using CAC11219 at dilution of 1:100
Immunofluorescence staining of Hela cells with CAC11219 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating POLDIP3 in Jurkat whole cell lysate; , Lane 1: Rabbit control IgG instead in Jurkat whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11219 (8µg) + Jurkat whole cell lysate (500µg); , Lane 3: Jurkat whole cell lysate (10µg);

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