Plaur Polyclonal Antibody, Cat#CAC07382
IHC image of CAC07382 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC07382 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; , All lanes: PLAUR antibody at 4µg/ml; , Lane 1: Mouse brain tissue; , Lane 2: A2780 whole cell lysate; , Lane 3: MCF-7 whole cell lysate; , Lane 4: Hela whole cell lysate; , Lane 5: Colo320 whole cell lysate; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 37, 32, 33 kDa; , Observed band size: 37 kDa
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Plaur Polyclonal Antibody, Cat#CAC07382 IHC image of CAC07382 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CAC07382 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western blot; , All lanes: PLAUR antibody at 4µg/ml; , Lane 1: Mouse brain tissue; , Lane 2: A2780 whole cell lysate; , Lane 3: MCF-7 whole cell lysate; , Lane 4: Hela whole cell lysate; , Lane 5: Colo320 whole cell lysate; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 37, 32, 33 kDa; , Observed band size: 37 kDa

Ultra Sensitive IHC

Plaur Polyclonal Antibody

BiomatikSKU: CAC07382-100ug
Size: 100ug
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IHC image of CAC07382 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC07382 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; , All lanes: PLAUR antibody at 4µg/ml; , Lane 1: Mouse brain tissue; , Lane 2: A2780 whole cell lysate; , Lane 3: MCF-7 whole cell lysate; , Lane 4: Hela whole cell lysate; , Lane 5: Colo320 whole cell lysate; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 37, 32, 33 kDa; , Observed band size: 37 kDa

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