Pih1D1 Polyclonal Antibody, Cat#CAC09919
Immunohistochemistry of paraffin-embedded human placenta tissue using CAC09919 at dilution of 1:100
Immunofluorescence staining of Hela cells cells with CAC09919 at 1: 200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Immunoprecipitating PIH1D1 in Hela whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC09919 (6ug) + Hela whole cell lysate (500ug); , Lane 3: Hela whole cell lysate (10ug)
Western Blot; , Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; , All lanes: PIH1D1 antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 33, 20, 22 kDa; , Observed band size: 39 kDa
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Pih1D1 Polyclonal Antibody, Cat#CAC09919 Immunohistochemistry of paraffin-embedded human placenta tissue using CAC09919 at dilution of 1:100 Immunofluorescence staining of Hela cells cells with CAC09919 at 1: 200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) . Immunoprecipitating PIH1D1 in Hela whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC09919 (6ug) + Hela whole cell lysate (500ug); , Lane 3: Hela whole cell lysate (10ug) Western Blot; , Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; , All lanes: PIH1D1 antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 33, 20, 22 kDa; , Observed band size: 39 kDa

Ultra Sensitive IHC

Pih1D1 Polyclonal Antibody

BiomatikSKU: CAC09919-100ug
Size: 100ug
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Immunohistochemistry of paraffin-embedded human placenta tissue using CAC09919 at dilution of 1:100
Immunofluorescence staining of Hela cells cells with CAC09919 at 1: 200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Immunoprecipitating PIH1D1 in Hela whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC09919 (6ug) + Hela whole cell lysate (500ug); , Lane 3: Hela whole cell lysate (10ug)
Western Blot; , Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; , All lanes: PIH1D1 antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 33, 20, 22 kDa; , Observed band size: 39 kDa

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