Phf6 Polyclonal Antibody

BiomatikSKU: CAC08722-100ug

Size: 100ug
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Technical Documents

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Immunofluorescence staining of Hela cells with CAC08722 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; ,Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; ,All lanes: PHF6 antibody at 2.8µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 42, 36, 38 kDa; ,Observed band size: 42 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CAC08722 at dilution of 1:100
Immunoprecipitating PHF6 in Jurkat whole cell lysate; , Lane 1: Rabbit control IgG instead in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC08722 (8µg) + Jurkat whole cell lysate (500µg); , Lane 3: Jurkat whole cell lysate (10µg)

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