PHB Polyclonal Antibody, Cat#CAC13157
IHC image of CAC13157 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot, Positive WB detected in: Raji whole cell lysate, All lanes: PHB antibody at 4.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 30, 18 kDa, Observed band size: 30 kDa
Immunoprecipitating PHB in HEK293 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13157 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13157 (8ug) + HEK293 whole cell lysate (500ug), Lane 3: HEK293 whole cell lysate (10ug)
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PHB Polyclonal Antibody, Cat#CAC13157 IHC image of CAC13157 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Western Blot, Positive WB detected in: Raji whole cell lysate, All lanes: PHB antibody at 4.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 30, 18 kDa, Observed band size: 30 kDa Immunoprecipitating PHB in HEK293 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13157 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13157 (8ug) + HEK293 whole cell lysate (500ug), Lane 3: HEK293 whole cell lysate (10ug)

Ultra Sensitive IHC

PHB Polyclonal Antibody

BiomatikSKU: CAC13157-100ug
Size: 100ug
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IHC image of CAC13157 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot, Positive WB detected in: Raji whole cell lysate, All lanes: PHB antibody at 4.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 30, 18 kDa, Observed band size: 30 kDa
Immunoprecipitating PHB in HEK293 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13157 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13157 (8ug) + HEK293 whole cell lysate (500ug), Lane 3: HEK293 whole cell lysate (10ug)

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Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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