Ultra Sensitive IHC

Pex26 Polyclonal Antibody

BiomatikSKU: CAC11399-100ug

Size: 100ug
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Description

Technical Documents

Images

Western Blot; Positive WB detected in: K562 whole cell lysate; All lanes: PEX26 antibody at 3.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34, 29 kDa; Observed band size: 34 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating PEX26 in K562 whole cell lysate; , Lane 1: Rabbit control IgG instead in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11399 (6µg) + K562 whole cell lysate (500µg); , Lane 3: K562 whole cell lysate (20µg)

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