Ultra Sensitive IHC

Pecam1 Polyclonal Antibody

BiomatikSKU: CAC07879-100ug

Size: 100ug
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Description

Technical Documents

Images

Western Blot; Positive WB detected in: Raji whole cell lysate, Mouse kidney tissue, Rat heart tissue, Rat lung tissue; All lanes: PECAM1 antibody at 2.7µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 83, 81, 80, 82 kDa; Observed band size: 83 kDa;
IHC image diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of HepG2 cells using CAC07879 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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