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Western Blot; Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate ; All lanes PD-L2 antibody at 1:2000; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 31,21 KDa; Observed band size: 45-50 KDa; Exposure time:5min
Western Blot; ,Positive WB detected in: Hela whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg,All lanes: PD-L2 antibody at 1:2000; ,Secondary; ,Goat polyclonal to mouse IgG at 1/50000 dilution; ,Predicted band size: 31,21 KDa; ,Observed band size: 45-50 KDa; ,Exposure time:5min
IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.,

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