Ultra Sensitive IHC

Pd-L1 Monoclonal Antibody

BiomatikSKU: CAC10271-100ul

Size: 100ul
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Description

Technical Documents

Images

Western Blot; Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate; All lanes: PD-L1 antibody at 1:2500; Secondary; Goat polyclonal to Mouse IgG at 1/10000 dilution; Predicted band size: 33 kDa; Observed band size: 55 kDa;
Western Blot; ,Positive WB detected in: Hela whole cell lysate; ,All lanes: PD-L1 antibody at 1:1000, 1:2000, 1:4000; ,Secondary; ,Goat polyclonal to Mouse IgG at 1/10000 dilution; ,Predicted band size: 33 kDa; ,Observed band size: 55 kDa;
IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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