OTX2 Polyclonal Antibody, Cat#CAC13854
Western Blot, All lanes: OTX2 antibody at 4ug/ml, Lane 1: Jurkat whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: Mouse lung tissue, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution, Predicted band size: 32, 33 kDa, Observed band size: 32 kDa
IHC image of CAC13854 diluted at 1:250 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC13854 diluted at 1:250 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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OTX2 Polyclonal Antibody, Cat#CAC13854 Western Blot, All lanes: OTX2 antibody at 4ug/ml, Lane 1: Jurkat whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: Mouse lung tissue, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution, Predicted band size: 32, 33 kDa, Observed band size: 32 kDa IHC image of CAC13854 diluted at 1:250 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CAC13854 diluted at 1:250 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Ultra Sensitive IHC

OTX2 Polyclonal Antibody

BiomatikSKU: CAC13854-100ug
Size: 100ug
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Technical Documents

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Images

Western Blot, All lanes: OTX2 antibody at 4ug/ml, Lane 1: Jurkat whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: Mouse lung tissue, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution, Predicted band size: 32, 33 kDa, Observed band size: 32 kDa
IHC image of CAC13854 diluted at 1:250 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC13854 diluted at 1:250 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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