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Western Blot; Positive WB detected in: U251 whole cell lysate, U87 whole cell lysate; All lanes: OTUD3 antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 46 kDa; Observed band size: 46 kDa;
Immunofluorescence staining of U251 cells with CAC11672 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Safety Data Sheet