OFD1 Polyclonal Antibody, Cat#CAC13142
IHC image of CAC13142 diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western BlotPositive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysateAll lanes: OFD1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 117, 43, 112 kDaObserved band size: 117 kDa
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OFD1 Polyclonal Antibody, Cat#CAC13142 IHC image of CAC13142 diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Western BlotPositive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysateAll lanes: OFD1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 117, 43, 112 kDaObserved band size: 117 kDa

Ultra Sensitive IHC

OFD1 Polyclonal Antibody

BiomatikSKU: CAC13142-100ul
Size: 100ul
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IHC image of CAC13142 diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western BlotPositive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysateAll lanes: OFD1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 117, 43, 112 kDaObserved band size: 117 kDa

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We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

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