NUP98 Polyclonal Antibody, Cat#CAC13141
Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, All lanes: NUP98 antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 198, 188, 98, 97, 196, 187 kDa, Observed band size: 105 kDa
Immunofluorescence staining of A549 cells with CAC13141 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating NUP98 in Jurkat whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13141 in Jurkat whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13141 (8ug) + Jurkat whole cell lysate (500ug), Lane 3: Jurkat whole cell lysate (20ug)
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NUP98 Polyclonal Antibody, Cat#CAC13141 Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, All lanes: NUP98 antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 198, 188, 98, 97, 196, 187 kDa, Observed band size: 105 kDa Immunofluorescence staining of A549 cells with CAC13141 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating NUP98 in Jurkat whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13141 in Jurkat whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13141 (8ug) + Jurkat whole cell lysate (500ug), Lane 3: Jurkat whole cell lysate (20ug)

NUP98 Polyclonal Antibody

BiomatikSKU: CAC13141-100ug
Size: 100ug
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Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, All lanes: NUP98 antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 198, 188, 98, 97, 196, 187 kDa, Observed band size: 105 kDa
Immunofluorescence staining of A549 cells with CAC13141 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating NUP98 in Jurkat whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13141 in Jurkat whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13141 (8ug) + Jurkat whole cell lysate (500ug), Lane 3: Jurkat whole cell lysate (20ug)

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