NUP62 Polyclonal Antibody, Cat#CAC13140
Western Blot, Positive WB detected in: Jurkat whole cell lysate, RAW264.7 whole cell lysate, PC-3 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, All lanes: NUP62 antibody at 3.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 54 kDa, Observed band size: 62 kDa
IHC image of CAC13140 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating NUP62 in A549 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13140 in A549 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC13140 (6ug) + A549 whole cell lysate (1mg), Lane 3: A549 whole cell lysate (20ug)
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NUP62 Polyclonal Antibody, Cat#CAC13140 Western Blot, Positive WB detected in: Jurkat whole cell lysate, RAW264.7 whole cell lysate, PC-3 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, All lanes: NUP62 antibody at 3.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 54 kDa, Observed band size: 62 kDa IHC image of CAC13140 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating NUP62 in A549 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13140 in A549 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC13140 (6ug) + A549 whole cell lysate (1mg), Lane 3: A549 whole cell lysate (20ug)

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NUP62 Polyclonal Antibody

BiomatikSKU: CAC13140-100ug
Size: 100ug
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Western Blot, Positive WB detected in: Jurkat whole cell lysate, RAW264.7 whole cell lysate, PC-3 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, All lanes: NUP62 antibody at 3.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 54 kDa, Observed band size: 62 kDa
IHC image of CAC13140 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating NUP62 in A549 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13140 in A549 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC13140 (6ug) + A549 whole cell lysate (1mg), Lane 3: A549 whole cell lysate (20ug)

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