Western Blot, Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, All lanes: NUMA1 antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 239, 237, 202, 201, 110 kDa, Observed band size: 239 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CAC12913 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CAC12913 at dilution of 1:100
Immunofluorescence staining of MCF-7 cells with CAC12913 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).