Ultra Sensitive IHC

NT5E Polyclonal Antibody

BiomatikSKU: CAC15737-100ul

Size: 100ul
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Technical Documents

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Western Blot, Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, HUVEC whole cell lysate, All lanes: NT5E antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 64, 58 kDa, Observed band size: 64 kDa
IHC image of CAC15737 diluted at 1:30 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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