Immunohistochemistry of paraffin-embedded human endometrial cancer using CAC14441 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CAC14441 at dilution of 1:100
Immunofluorescence staining of Hela cells with CAC14441 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating NRBP2 in A549 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC14441 in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14441 (6ug) + A549 whole cell lysate (500ug), Lane 3: A549 whole cell lysate (10ug)