Western Blot; Positive WB detected in: HepG2 whole cell lysate; All lanes: NR3C1 antibody at 3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 86, 82, 65, 61, 83, 79, 77, 76, 52, 51, 50 kDa; Observed band size: 86 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CAC11199 at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CAC11199 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating NR3C1 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000); , Lane 2: CAC11199 (8µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg)