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Western Blot, Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, All lanes: NFKBIA antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 36 kDa, Observed band size: 36 kDa
IHC image of CAC15883 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cells with CAC15883 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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