Nat10 Polyclonal Antibody, Cat#CAC09727
IHC image of CAC09727 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating NAT10 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC09727 (6µg) + Hela whole cell lysate (1mg); , Lane 3: Hela whole cell lysate (20µg);
Western Blot; , Positive WB detected in: Hela whole cell lysate; , All lanes:NAT10 antibody at 7µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 116, 108 kDa; , Observed band size: 116 kDa;
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Nat10 Polyclonal Antibody, Cat#CAC09727 IHC image of CAC09727 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating NAT10 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC09727 (6µg) + Hela whole cell lysate (1mg); , Lane 3: Hela whole cell lysate (20µg); Western Blot; , Positive WB detected in: Hela whole cell lysate; , All lanes:NAT10 antibody at 7µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 116, 108 kDa; , Observed band size: 116 kDa;

Ultra Sensitive IHC

Nat10 Polyclonal Antibody

BiomatikSKU: CAC09727-100ug
Size: 100ug
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IHC image of CAC09727 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating NAT10 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC09727 (6µg) + Hela whole cell lysate (1mg); , Lane 3: Hela whole cell lysate (20µg);
Western Blot; , Positive WB detected in: Hela whole cell lysate; , All lanes:NAT10 antibody at 7µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 116, 108 kDa; , Observed band size: 116 kDa;

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