Ultra Sensitive IHC

NAGPA Polyclonal Antibody

BiomatikSKU: CAC15520-100ug

Size: 100ug
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Technical Documents

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Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, All lanes: NAGPA antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 57, 53, 34 kDa, Observed band size: 53 kDa
IHC image of CAC15520 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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