Myo5A Polyclonal Antibody, Cat#CAC11285
Western Blot; Positive WB detected in: Mouse brain tissue; All lanes: MYO5A antibody at 7.3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 216, 213, 219 kDa; Observed band size: 216 kDa;
IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CAC11285 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; , Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate; , All lanes: MYO5A antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 216, 213, 219 kDa; , Observed band size: 216 kDa;
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Myo5A Polyclonal Antibody, Cat#CAC11285 Western Blot; Positive WB detected in: Mouse brain tissue; All lanes: MYO5A antibody at 7.3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 216, 213, 219 kDa; Observed band size: 216 kDa; IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CAC11285 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot; , Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate; , All lanes: MYO5A antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 216, 213, 219 kDa; , Observed band size: 216 kDa;

Ultra Sensitive IHC

Myo5A Polyclonal Antibody

BiomatikSKU: CAC11285-100ug
Size: 100ug
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Western Blot; Positive WB detected in: Mouse brain tissue; All lanes: MYO5A antibody at 7.3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 216, 213, 219 kDa; Observed band size: 216 kDa;
IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CAC11285 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; , Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate; , All lanes: MYO5A antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 216, 213, 219 kDa; , Observed band size: 216 kDa;

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