Mono-methyl-HIST1H4A (K59) Polyclonal Antibody

BiomatikSKU: CAC15459-100ul

Size: 100ul
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Technical Documents

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Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, All lanes: HIST1H4A antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunofluorescence staining of Hela cells with CAC15459 at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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