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Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15505) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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