Mono-methyl-HIST1H2BC (K12) Polyclonal Antibody, Cat#CAC15545
Western Blot, Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunocytochemistry analysis of CAC15545 diluted at 1:30 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15545) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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Mono-methyl-HIST1H2BC (K12) Polyclonal Antibody, Cat#CAC15545 Western Blot, Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa Immunocytochemistry analysis of CAC15545 diluted at 1:30 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15545) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Mono-methyl-HIST1H2BC (K12) Polyclonal Antibody

BiomatikSKU: CAC15545-100ul
Size: 100ul
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Western Blot, Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunocytochemistry analysis of CAC15545 diluted at 1:30 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15545) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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