Mono-methyl-HIST1H1C (K96) Polyclonal Antibody, Cat#CAC15264
Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, All lanes: HIST1H1C antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa
Immunofluorescence staining of Hela cells with CAC15264 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1C (CAC15264) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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Mono-methyl-HIST1H1C (K96) Polyclonal Antibody, Cat#CAC15264 Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, All lanes: HIST1H1C antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa Immunofluorescence staining of Hela cells with CAC15264 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1C (CAC15264) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Mono-methyl-HIST1H1C (K96) Polyclonal Antibody

BiomatikSKU: CAC15264-100ul
Size: 100ul
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Western Blot, Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, All lanes: HIST1H1C antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa
Immunofluorescence staining of Hela cells with CAC15264 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1C (CAC15264) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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