Mono-methyl-H1F0 (K101) Polyclonal Antibody, Cat#CAC15288
Western Blot, Positive WB detected in: HepG2 whole cell lysate, All lanes: H1F0 antibody at 1:50, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 21, 20 kDa, Observed band size: 21 kDa
Immunofluorescence staining of HepG2 cells with CAC15288 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H1F0 (CAC15288) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunocytochemistry analysis of CAC15288 diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Mono-methyl-H1F0 (K101) Polyclonal Antibody, Cat#CAC15288 Western Blot, Positive WB detected in: HepG2 whole cell lysate, All lanes: H1F0 antibody at 1:50, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 21, 20 kDa, Observed band size: 21 kDa Immunofluorescence staining of HepG2 cells with CAC15288 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H1F0 (CAC15288) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter. Immunocytochemistry analysis of CAC15288 diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Mono-methyl-H1F0 (K101) Polyclonal Antibody

BiomatikSKU: CAC15288-100ul
Size: 100ul
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Western Blot, Positive WB detected in: HepG2 whole cell lysate, All lanes: H1F0 antibody at 1:50, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 21, 20 kDa, Observed band size: 21 kDa
Immunofluorescence staining of HepG2 cells with CAC15288 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H1F0 (CAC15288) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunocytochemistry analysis of CAC15288 diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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