Ultra Sensitive IHC

Mmp3 Polyclonal Antibody

BiomatikSKU: CAC09204-100ug

Size: 100ug
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Technical Documents

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IHC image of CAC09204 diluted at 1:709 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot; ,Positive WB detected in: Mouse liver tissue, Mouse kidney tissue; ,All lanes: Mmp3 antibody at 2.8µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 54 kDa; ,Observed band size: 54 kDa

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