Mkln1 Polyclonal Antibody, Cat#CAC11524
Western Blot; Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Rat brain tissue; All lanes: MKLN1 antibody at 2.7µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 85, 61 kDa; Observed band size: 85 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Mkln1 Polyclonal Antibody, Cat#CAC11524 Western Blot; Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Rat brain tissue; All lanes: MKLN1 antibody at 2.7µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 85, 61 kDa; Observed band size: 85 kDa IHC image diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Ultra Sensitive IHC

Mkln1 Polyclonal Antibody

BiomatikSKU: CAC11524-100ug
Size: 100ug
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Western Blot; Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Rat brain tissue; All lanes: MKLN1 antibody at 2.7µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 85, 61 kDa; Observed band size: 85 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

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