Western Blot, All lanes: MAOB antibody at 9ug/ml, Lane 1: Mouse liver tissue, Lane 2: HepG2 whole cell lysate, Lane 3: A549 whole cell lysate, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution, Predicted band size: 59, 47 kDa, Observed band size: 59, 27 kDa
Immunohistochemistry of paraffin-embedded mouse liver tissue using CAC13813 at dilution of 1:50
IHC image of CAC13813 diluted at 1:300 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.