Western Blot, Positive WB detected in: Rat kidney tissue, All lanes: MAN2B2 antibody at 3.4ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 114, 108 kDa, Observed band size: 114 kDa
Western Blot, Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, All lanes: MAN2B2 antibody at 1.4ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 114, 108 kDa, Observed band size: 135 kDa
IHC image of CAC15154 diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.