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Western Blot; Positive WB detected in: HEK293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate; All lanes: LZTFL1 antibody at 3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 35, 33, 30 kDa; Observed band size: 35, 50 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CAC07752 at dilution of 1:100
Immunofluorescent analysis of U251 cells using CAC07752 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating LZTFL1 in HepG2 whole cell lysate; , Lane 1: Rabbit control IgG instead of (1µg) instead in HepG2 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000); , Lane 2: CAC07752 (4µg) + HepG2 whole cell lysate (500µg); , Lane 3: HepG2 whole cell lysate (20µg)

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