Western Blot; Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate, Rat brain tissue; All lanes: LRTM2 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa
IHC image diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot; , Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, 293 whole cell lysate; , All lanes: LRTM2 antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 42 kDa; , Observed band size: 42 kDa