Ultra Sensitive IHC

Lpar4 Polyclonal Antibody

BiomatikSKU: CAC08597-100ug

Size: 100ug
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Description

Technical Documents

Images

IHC image of CAC08597 diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot; ,All lanes: LPAR4 antibody at 4µg/ml + Mouse kidney tissue; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 42 kDa; ,Observed band size: 42 kDa
Immunofluorescent analysis of HepG2 cells using CAC08597 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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