Lag3 Polyclonal Antibody, Cat#CAC08586
Western Blot; Positive WB detected in: HepG2 whole cell lysate; All lanes: LAG3 antibody at 4.9µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 58, 40 kDa; Observed band size: 58 kDa
Immunofluorescence staining of HepG2 cells with CAC08586 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Lag3 Polyclonal Antibody, Cat#CAC08586 Western Blot; Positive WB detected in: HepG2 whole cell lysate; All lanes: LAG3 antibody at 4.9µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 58, 40 kDa; Observed band size: 58 kDa Immunofluorescence staining of HepG2 cells with CAC08586 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Lag3 Polyclonal Antibody

BiomatikSKU: CAC08586-100ug
Size: 100ug
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Western Blot; Positive WB detected in: HepG2 whole cell lysate; All lanes: LAG3 antibody at 4.9µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 58, 40 kDa; Observed band size: 58 kDa
Immunofluorescence staining of HepG2 cells with CAC08586 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

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