Lacz Monoclonal Antibody, Cat#CAC10272
Western Blot; Positive WB detected in Recombinant protein; All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000; Secondary; Goat polyclonal to Mouse IgG at 1/50000 dilution; Predicted band size: 117 kDa; Observed band size: 130 kDa;
Western Blot; ,Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng); ,All lanes: lacZ antibody at 1:2000; ,Secondary; ,Goat polyclonal to Mouse IgG at 1/50000 dilution; ,Predicted band size: 117 kDa; ,Observed band size: 130 kDa;
Immunofluorescence staining of 293T cells transfected with CAC10272 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
Immunoprecipitating lacZ in 293T transfected whole cell lysate ; , Lane 1: Mouse control IgG instead in 293T transfected whole cell lysate., Lane 2: CAC10272 (2µl) + 293T transfected whole cell lysate (500µg), Lane 3: 293T transfected whole cell lysate (20µg) ; , For western blotting, the blot was detected with CAC10272 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000;
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Lacz Monoclonal Antibody, Cat#CAC10272 Western Blot; Positive WB detected in Recombinant protein; All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000; Secondary; Goat polyclonal to Mouse IgG at 1/50000 dilution; Predicted band size: 117 kDa; Observed band size: 130 kDa; Western Blot; ,Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng); ,All lanes: lacZ antibody at 1:2000; ,Secondary; ,Goat polyclonal to Mouse IgG at 1/50000 dilution; ,Predicted band size: 117 kDa; ,Observed band size: 130 kDa; Immunofluorescence staining of 293T cells transfected with CAC10272 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody Immunoprecipitating lacZ in 293T transfected whole cell lysate ; , Lane 1: Mouse control IgG instead in 293T transfected whole cell lysate., Lane 2: CAC10272 (2µl) + 293T transfected whole cell lysate (500µg), Lane 3: 293T transfected whole cell lysate (20µg) ; , For western blotting, the blot was detected with CAC10272 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000;

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Lacz Monoclonal Antibody

BiomatikSKU: CAC10272-100ul
Size: 100ul
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Western Blot; Positive WB detected in Recombinant protein; All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000; Secondary; Goat polyclonal to Mouse IgG at 1/50000 dilution; Predicted band size: 117 kDa; Observed band size: 130 kDa;
Western Blot; ,Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng); ,All lanes: lacZ antibody at 1:2000; ,Secondary; ,Goat polyclonal to Mouse IgG at 1/50000 dilution; ,Predicted band size: 117 kDa; ,Observed band size: 130 kDa;
Immunofluorescence staining of 293T cells transfected with CAC10272 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
Immunoprecipitating lacZ in 293T transfected whole cell lysate ; , Lane 1: Mouse control IgG instead in 293T transfected whole cell lysate., Lane 2: CAC10272 (2µl) + 293T transfected whole cell lysate (500µg), Lane 3: 293T transfected whole cell lysate (20µg) ; , For western blotting, the blot was detected with CAC10272 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000;

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Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

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We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

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