Western blot; All lanes: KYNU antibody at 1.44µg/ml; Lane 1: A549 whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: Hela whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 53, 35 kDa; Observed band size: 53 kDa;
Immunohistochemistry of paraffin-embedded human liver tissue using CAC10737 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CAC10737 at dilution of 1:100
Immunoprecipitating KYNU in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead of (1µg) instead in Hela whole cell lysate., For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000); , Lane 2: CAC10737 (4µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg);