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Western Blot, Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate, All lanes: KY antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 64, 42, 73, 76 kDa, Observed band size: 64 kDa
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CAC15015 at dilution of 1:100
Immunofluorescence staining of Hela cells with CAC15015 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Safety Data Sheet