Krt8 Polyclonal Antibody

BiomatikSKU: CAC07095-100ug

Size: 100ug
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Technical Documents

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Immunofluorescence staining of Hela cells with CAC07095 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: KRT8 antibody at 2µg/ml; ,Lane 1: A549 whole cell lysate; ,Lane 2: MCF-7 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 54, 57 kDa; ,Observed band size: 54 kDa
Immunohistochemistry of paraffin-embedded human lung cancer using CAC07095 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CAC07095 at dilution of 1:100

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