Western blot; All lanes: KHKantibody at 3.6µg/ml; Lane 1: Mouse liver tissue; Lane 2: Mouse kidney tissue; Lane 3: Rat kidney tissue; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 33 kDa; Observed band size: 33 kDa;
Immunohistochemistry of paraffin-embedded human liver tissue using CAC10774 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CAC10774 at dilution of 1:100
Immunoprecipitating KHK in Mouse liver tissue; , Lane 1: Rabbit control IgG instead in Mouse liver tissue., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC10774 (8µg) + Mouse liver tissue (500µg); , Lane 3: Mouse liver tissue (10µg);