Western Blot; Positive WB detected in: SH-SY5Y whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate; All lanes: ITGB1BP1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 22, 17 kDa; Observed band size: 22 kDa
IHC image diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.