ITCH Polyclonal Antibody

BiomatikSKU: CAC15758-100ul

Size: 100ul
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Western Blot, Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, All lanes: ITCH antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 103, 99, 87 kDa, Observed band size: 103 kDa
Immunofluorescence staining of HepG2 cells with CAC15758 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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