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Irx4 Polyclonal Antibody

BiomatikSKU: CAC11175-100ug

Size: 100ug
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Description

Technical Documents

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Western Blot; Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, Colo320 whole cell lysate; All lanes: IRX4 antibody at 3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 55, 58 kDa; Observed band size: 55 kDa
Immunofluorescence staining of MCF-7 cells with CAC11175 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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